Biocompatible Materials Based on Self-Assembling Peptides on Ti25Nb10Zr Alloy: Molecular Structure and Organization Investigated by Synchrotron Radiation Induced Techniques
نویسندگان
چکیده
In this work, we applied advanced Synchrotron Radiation (SR) induced techniques to the study of the chemisorption of the Self Assembling Peptide EAbuK16, i.e., H-Abu-Glu-Abu-Glu-Abu-Lys-Abu-Lys-Abu-Glu-Abu-Glu-Abu-Lys-Abu-Lys-NH₂ that is able to spontaneously aggregate in anti-parallel β-sheet conformation, onto annealed Ti25Nb10Zr alloy surfaces. This synthetic amphiphilic oligopeptide is a good candidate to mimic extracellular matrix for bone prosthesis, since its β-sheets stack onto each other in a multilayer oriented nanostructure with internal pores of 5-200 nm size. To prepare the biomimetic material, Ti25Nb10Zr discs were treated with aqueous solutions of EAbuK16 at different pH values. Here we present the results achieved by performing SR-induced X-ray Photoelectron Spectroscopy (SR-XPS), angle-dependent Near Edge X-ray Absorption Fine Structure (NEXAFS) spectroscopy, FESEM and AFM imaging on Ti25Nb10Zr discs after incubation with self-assembling peptide solution at five different pH values, selected deliberately to investigate the best conditions for peptide immobilization.
منابع مشابه
A Review of the Applications of Synchrotron Radiation in Archaeological Sciences
The scientific research regarding investigation, characterization and protection of the archeological specimens is manifested through a notable participation of multidisciplinary subjects and experts, scientists and archeometrists. One of the main principals which are considered by archaeometrists in the study of the precious specimens is the utilizing nondestructive methods. As an example, in ...
متن کاملSelf-assembly of keratin peptides: Its implication on the performance of electrospun PVA nanofibers
Drawing inspiration from the field of designer self-assembling materials, this work is aimed to focus on the self-assembling nature of extracted peptides. Hair keratin, a proteinacious reject in tanning industry has been chosen since they have been extracted and used for wide range of applications. Keratin source was subjected to five hydrolysis treatments (viz., sulphitolysis, β-mercaptoethano...
متن کاملRational Molecular Design of Complementary Self-Assembling Peptide Hydrogels
Rational molecular design of self- assembling peptide-based materials that spontaneously form self-supporting hydrogels shows potential in many healthcare applications. Binary peptides based on complementary charged sequences are developed, and the use of biophysical analysis and cell-based studies highlights that the charged interactions can influence the properties of peptide materials and ul...
متن کاملThe effect of polylactic acid support in stability and electrical field of heterocyclic coupled hexa peptide nano systems: A novel strateu to drug delivery
Biological materials. recently. are the building blocks of several self-assembling peptide and protein systems.The main challenge In molecular self-assembly is to design molecular building blocks that can undergospontaneous organization. These cyche peptides were produced by an alternating fl'ell number of D- and Laminoacids.which interact through non-covalent interactions co an array of selfas...
متن کاملDense surface functionalization using peptides that recognize differences in organized structures of self-assembling nanomaterials.
We obtained novel peptides that selectively bind to self-assembling peptide nanomaterials from a random peptide library displayed on phages. Affinity-dependent peptide screening gave phage clones displaying peptides with selective affinities against two kinds of highly networked nanofibers constructed of β-sheet peptides. Both peptide nanofibers have similar primary structures. Binding analyses...
متن کامل